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Bench Marks
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A blog by David Crotty
Executive Editor of CSH Protocols
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BAP: Bimolecular Affinity Purification
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The tandem affinity purification (TAP) procedure was pioneered in yeast for the purpose of purifying and characterizing protein complexes and has since been adapted for use in many organisms, including mammalian systems. The TAP procedure involves two sequential affinity purification steps to avoid non-specific protein interactions, a common problem in identifying proteins in complexes. [...]
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