Archived Protocols Preparation of Biodegradable Nanoparticles and Their Use in Transfection Culturing Trophoblast Stem (TS) Cell Lines In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry Genome Modification by Inclusion of loxP Transgene Sequences Preparation of Reversed-Phase Microcolumns Fate-Mapping Technique: Targeted Whole-Embryo Electroporation of DNA Constructs into the Germ Layers of Mouse Embryos 7-7.5 Days Post-coitum Generation of Transgenic Xenopus laevis Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatography Genotyping by Oligonucleotide Ligation Assay (OLA) Histological Preparation of Embryonic and Adult Zebrafish Eyes Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells Protein Interactions Captured by Chemical Cross-linking: One-Step Cross-linking with Formaldehyde Chromatin Immunoprecipitation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications Analysis of the Topology of Protein Complexes Using Cross-Linking and Mass Spectrometry PCR-Based Analysis of Immunoprecipitated Chromatin Production of dsRNA for RNAi in Avian Embryos Preparation and Use of an Integrated Microcapillary HPLC Column and ESI Device for Proteomic Analysis General Method for MALDI-MS Analysis of Proteins and Peptides Yeast Chromatin Immunoprecipitation (ChIP) Assay